Axe Three Waters model, co-fund infrastructure projects – Stuff.co.nz

OPINION: There’s enormous merit in pursuing the benefits of economies of scale, to help drive efficiencies and blitz the cost burden of duplicated administrative structures.
That’s primarily why I support the looming dissolution of our 20 district health boards and their perverse “postcode lottery” health outcomes.
However, the Government’s Three Waters reform programme, which would see all 67 councils relinquish their water infrastructure assets to four mega-regional water entities, looks dubious at best.
The reform agenda’s attempt to court public support has been blighted by a profligate, sensationalist and infantilising advertising blitz. It doesn’t pass the sniff test.
READ MORE:
* Three Waters reform pushed ‘at alarming rate’, Timaru District mayor says
* Average Ashburton water bill could rise to $9000 without reforms – report
* Canterbury districts stand to gain more from Government water reforms than Christchurch
* South Canterbury mayors raise concerns over three waters reform
* Water reforms hit an expensive snag, as cost estimate rises to $185b

The misleading advertising campaign, which clearly implies that our drinking water is gravely polluted and can’t be trusted, has played into the hands of the critics, who are increasingly perturbed by the government’s centralisation and co-governance agenda.
In Canterbury, the lack of appetite for these “asset-grabbing” reforms is resounding. The Waimakariri and Selwyn district councils have already stated it’s unlikely that they’ll voluntarily opt in.
Christchurch seems headed in the same direction, highly sceptical about the underlying financial assumptions and touted benefits being spruiked by the State.
Currently, the average Christchurch residential ratepayer pays $920 annually towards the city’s water infrastructure and services, accounting for roughly 30 per cent of the annual residential rates bill. If we are to believe the Government’s figures, that average water services charge will rise to $2720 in 2051, without reform.
However, if we opt in to the reforms, the average annual cost can be contained to $1640, in 30 years’ time.
Frankly, I don’t buy it. Just look at the average residential rates track in the past 10 years. Since 2011, my overall annual rates bill has rocketed by 104 per cent.
Three Waters rates charges have risen by nearly 300 per cent over the same period, as Christchurch tries to play catch-up on its ageing infrastructure.
Yet, the Government would have us believe, 30 years from now, we’ll only be paying 79 per cent more for Christchurch water rates than we do now, if we pony up to the regional water entity.
If we go it alone, they project the increase will balloon by 200 per cent. Meanwhile, Auckland ratepayers are being sold the carrot that their 2051 average water bill will only be $800, compared with $1640 in Christchurch. How is that equitable?
At the other end of the spectrum, councils with the smallest ratepayer bases, like Hurunui, Mackenzie and Waimate, are being promised that nearly $7000 will be shaved off their annual household water rates bills.
The city council is currently evaluating the veracity of the Government’s financial assumptions, during the eight-week engagement process. But after watching the full presentation to councillors last week, which resembled a mass convention of doubting Thomases, I’d be staggered if Christchurch ultimately opts in.
(As an aside, any hopes that Christchurch can successfully apply for an exemption to remove chlorine from our drinking supply, under the new Water Services Bill, looks remote.)
Former mayor Vicki Buck makes a compelling point, that if water assets were prised away from councils, it would destroy the ability of councils to co-ordinate multiple works, when digging up a road.
These convoluted water entities and their multiple governance and management layers will undeniably erode local control and accountability. There’s a huge risk that after councils surrender their water infrastructure, which also includes wetlands, swales and flood basins, these entities don’t actually deliver real savings and instead morph into bloated bureaucracies, with messy cross-subsidising between neighbouring regions.
Local Government Minister Nanaia Mahuta has incongruously boasted that these entities will create 9000 new jobs! How is that an efficiency-boosting model?
Following the hasty engagement process and any semblance of public consultation, if councils en masse opt out of the process, will these reforms be dead in the water, or will the Government make them mandatory?
Such an outrageous act of state overreach would rightly be viewed as an abuse. Surely the Government would resist such an incendiary muscle-flex.
I believe a preferable alternative would be to axe this four-entity model and instead propose directly co-funding water infrastructure projects, particularly for councils with low ratepayer bases, in partnership with local government. The current 50-50 funding arrangement that governs roading projects lights the way.
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Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering – pnas.org

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Edited by Arthur Karlin, Columbia University, College of Physicians and Surgeons, New York, NY, and approved July 26, 2021 (received for review April 28, 2021)
Ligand-gated ion channels are membrane proteins that cycle rapidly between open and closed forms, a process called gating. The structural basis of gating is foundational to receptor biophysics, cellular physiology, and drug design; however, it remains unclear how experimental X-ray structures correspond to solution behavior. Here, we test the solution structure of a model ion channel, GLIC, based on small-angle neutron scattering and molecular simulations under conditions that favor closed or open states. We find that closed-state conditions correspond well to closed X-ray structures, while open-state conditions implicate intermediate or mixed open/closed structures. These results elucidate the states sampled during ion channel gating, and the utility of neutron scattering combined with simulations to distinguish subtle shifts in membrane protein structure.
Pentameric ligand-gated ion channels undergo subtle conformational cycling to control electrochemical signal transduction in many kingdoms of life. Several crystal structures have now been reported in this family, but the functional relevance of such models remains unclear. Here, we used small-angle neutron scattering (SANS) to probe ambient solution-phase properties of the pH-gated bacterial ion channel GLIC under resting and activating conditions. Data collection was optimized by inline paused-flow size-exclusion chromatography, and exchanging into deuterated detergent to hide the micelle contribution. Resting-state GLIC was the best-fit crystal structure to SANS curves, with no evidence for divergent mechanisms. Moreover, enhanced-sampling molecular-dynamics simulations enabled differential modeling in resting versus activating conditions, with the latter corresponding to an intermediate ensemble of both the extracellular and transmembrane domains. This work demonstrates state-dependent changes in a pentameric ion channel by SANS, an increasingly accessible method for macromolecular characterization with the coming generation of neutron sources.
Pentameric ligand-gated ion channels (pLGICs) mediate electrochemical signal transduction in most kingdoms of life. In metazoa, these receptors populate postsynaptic membranes, where they open upon binding neurotransmitters to control the firing of action potentials; accordingly, human pLGICs are targets of anesthetic, anxiolytic, and other important classes of drugs (1). Several prokaryotic pLGICs have also been identified, possibly involved in chemotaxis or quorum sensing (2). Despite their physiological significance, our picture of the pLGIC gating landscape is still incomplete, and we only have limited knowledge, e.g., about the structural dynamics of conformations at room temperature or intermediate states, both of which are essential for understanding of fundamental channel biophysics as well as potential drug development.
The proton-gated cation channel GLIC, from the prokaryote Gloeobacter violaceus, has been a popular model system for structure–function studies in this superfamily (3); it accounts for 40% of all pLGIC structures in the Protein Data Bank (PDB). This channel is pH gated, and its structure has been solved at both high and low pH in apparent closed and open states, respectively (47). A static structural state is considered functionally open if the pore of the structure could conduct relevant ions; otherwise, it is considered a functionally closed state. These, however, are not necessarily the major physiological open and closed states. A further complication is that generally in pLGICs there are at least two closed physiological states—the resting state and the desensitized state—and a nonconducting conformation of the pore could indicate either of these states. Additional states have been determined in the presence of engineered mutations or lipids, possibly representing gating intermediates (812). Each channel is composed of five identical subunits, each of which contains an extracellular domain (ECD) consisting of 10 β-strands, and a transmembrane domain (TMD) of four α-helices (Fig. 1A). Relative to the closed structure, apparent open structures of GLIC undergo contraction in the ECD toward the central conduction pathway, as well as outward tilting of the pore-lining helices (6) (Fig. 1B). Recent structures of eukaryotic homologs including nAChR, GlyR, and 5-HT3A receptors indicate similar gating transitions, although the specific geometries and amino acid contacts are slightly different (1317).
Proposed structures and experimental approach for characterizing solution-phase GLIC by SANS. (A) Extracellular view of GLIC (PDB ID 4NPQ, closed), and two subunits seen from the membrane. The ECD in each subunit contains 10 β-strands, and the TMD four helices. Constriction points are labeled with the common prime notation for the pore lining helix of pLGICs. (B) Pore profiles calculated using Hole (18) for closed GLIC (PDB 4NPQ, dark blue), open GLIC (PDB 4HFI, light blue), tightly closed ELIC (PDB 3RQU, orange), and wide open sTeLIC (PDB 6FL9, red). Relative to the closed state, open GLIC has a wider pore at 9′ and 16′, while the top of the ECD has moved inward. ELIC and sTeLIC are similar in the ECD where both have a lower radius than GLIC; while the former is even more collapsed than closed GLIC in the upper part of the TMD, open sTeLIC has a wider pore than the other structures. (C) Schematic of the SEC-SANS experiment: A sample in H2O-based buffer with DDM detergent is loaded onto a size exclusion column running a D2O-based buffer with match-out deuterated DDM (d-DDM), resulting in the protein being exchanged to the D2O and d-DDM environment where the detergent is indistinguishable from the buffer in terms of neutron scattering. The SANS cell is in-line with the size exclusion chromatography, leading to measurements directly following size-based separation of the sample, which avoids sample aggregation.
Although X-ray structures of GLIC offer models both of stable and intermediate conformations, their correspondence to specific functional states on the gating pathway is still a matter of discussion. Structures of other bacterial family members suggest there are also alternative conformations in the pLGIC landscape, including a more tightly closed form of the pore in numerous structures of the plant pathogen channel ELIC (1921), and a dramatically expanded pore in the symbiote channel sTeLIC (22) (Fig. 1B). Both the ELIC and sTeLIC structures are contracted in the ECD compared to either closed or open GLIC, indicating further conformational diversity in this domain too. This could suggest some channels (e.g., GLIC) might be able to exist in conformations not yet observed experimentally, or that features such as crystal packing, experimental conditions, or the liquid nitrogen freezing might have biased the conformational distribution. Indeed, the vast majority of GLIC structures to date contain an activated ECD conformation; the few resting-state structures available were reported to notably lower resolution (6, 7). Biophysical techniques including electron paramagnetic resonance spectroscopy (11, 23) and atomic force microscopy (24) have suggested GLIC can sample additional states outside the crystalline context, emphasizing the importance of alternative structural approaches to model pLGIC conformational cycling in solution.
The solution structure of a protein can be probed using small-angle scattering, in which a sample’s distinctive scattering of X-rays or neutrons allows modeling of its population-average structure (Fig. 1C). Small-angle neutron scattering (SANS) was applied as early as 1979 to the muscle-type nicotinic acetylcholine receptor, a pLGIC extracted from native tissue of electric rays, yielding low-resolution predictions of molecular volume, radius of gyration, and overall size and shape (25). Thanks to the differential scattering of neutrons by isotopes of hydrogen, it is possible to hide the micellar environment of a membrane protein by matching the contrast of the detergent and the solvent. This has led to the development of match-out deuterated detergent (26) and nanodiscs (27), where the contrast is matched to pure heavy water (D2O). This strategy has recently been applied to probe AMPA receptor, ABC transporter, ATPase, photosystem I, and translocon structures in solution (26, 2830). However, given the relatively low resolution of SANS, the method has traditionally not been able to resolve minor structural changes between states due to, e.g., gating in ion channels in general and pLGICs in particular.
Another challenge is that membrane proteins can be highly prone to aggregation, which will lead to major distortion in the SANS spectrum, but this can be minimized with new methods that pass the sample directly to the neutron beam from a size-exclusion chromatography column (SEC-SANS) (31, 32) (Fig. 1C). This approach can be run continuously from column to measuring cell, or with pausing of the flow when the protein peak reaches the cell, enabling measurement at multiple detector distances in a single run. The continuous flow approach has been demonstrated (26, 32, 33), but there is little precedence in the literature for the paused-flow approach.
Here, we took advantage of SEC-SANS in match-out deuterated detergent to characterize solution structures of GLIC, which enabled us to measure scattering curves that exhibit slight differences between resting vs. activating conditions. By calculating theoretical scattering curves, we show that the closed-state X-ray conformation is the single experimental structure that best matches the experimental data, which excludes several alternative states. To better model the scattering data, we further applied enhanced-sampling molecular dynamics (MD) simulations to identify different ensembles of best-fit models in each condition, finding that an intermediate conformation or mixture of states best represents GLIC under activating conditions. This work demonstrates how SANS is able to resolve even subtle conformational changes between functional states of an ion channel at room temperature, the utility of SEC-SANS, and how the integration with MD-based enhanced sampling of flexibility in multiple states enables better matching of the activating conditions SANS measurements than any single experimental structure.
To investigate the solution structure of GLIC under resting and activating conditions, we first collected paused-flow SANS measurements of purified GLIC at pH 7.5 (resting conditions) and 3.0 (activating conditions), respectively. Guinier analysis to determine the radius of gyration revealed similar molecular radius (38.4 ± 0.2 Å at pH 7.5, and 38.3 ± 0.2 Å at pH 3.0) and weight estimates (194 kDa for both, with an expected error of 10% from the uncertainty in the protein concentration determination) (Fig. 2A). These values are in line with what is expected from the crystal structures (Rg of 37.9 Å for the closed structure, and 37.4 Å for the open), and from the amino acid sequence of the construct (182.7 kDa). For conformations this similar, it is not realistic to discriminate states directly from the overall radius of gyration. However, the scattering curves exhibited slight differences at Q-values around 0.10–0.12 Å−1—the region where differences are expected based on theoretical scattering curves calculated from crystal structures. A summary of the structural parameters calculated from the experimental curves is available in SI Appendix, Table S1.
Subtle effects of agonist (pH) revealed by paused-flow SEC-SANS. (A) SANS experimental data for GLIC measured using paused-flow SEC-SANS at resting (pH 7.5) and activating (pH 3.0) conditions, and the residual between the measured datasets. The overall shape of the experimental data are similar, but a difference is observed in the region Q∈[0.10,0.12] Å−1. The Guinier plot of the low Q-region is shown in the Inset, with the line representing the Guinier fit. (B) Pairwise distance distributions calculated from the closed GLIC model (blue), and derived from measurements at pH 7.5 using cuvette SANS (purple), continuous-flow SEC-SANS (standard SEC-SANS, pink), and paused-flow SEC-SANS (green). Both continuous-flow and paused-flow SEC-SANS avoid aggregation, unlike the cuvette measurement.
Concerning GLIC measured at pH 7.5, the paused-flow SEC-SANS arrangement—which enabled measurement of the same fraction at two detector distances—was compared with the classical cuvette-SANS configuration, and the continuous-flow SEC-SANS arrangement. Coupling inline size exclusion immediately prior to scattering measurements (SEC-SANS) eliminated the accumulation of low-order aggregates evident in the cuvette measurement. This is obvious in the pairwise distance distribution where there was close agreement between both SEC-SANS datasets and the distribution expected from the closed atomic model, while the cuvette measurement displayed a low amount of interatomic distances up to 150 Å (Fig. 2B).
To assess how the shape of solution-phase GLIC matches putative three-dimensional (3D) structures, we first compared our SANS data to scattering curves calculated from atomic models derived from previously reported X-ray structures: GLIC crystallized at pH 7.0 (closed, PDB ID 4NPQ) and 4.0 (open, PDB ID 4HFI), ELIC in the absence of agonist (tightly closed pore, PDB ID 3RQU), and sTeLIC in the presence of agonist (wide pore, PDB ID 6FL9). For the latter two comparisons, to ensure that model fits represented differences in large-scale conformation rather than local sequence differences between channels, we constructed homology models of GLIC based on template structures of ELIC and sTeLIC, respectively. Fitting of these theoretical spectra to the experimental measurements showed that the closed-state GLIC structure exhibited the best fit to the scattering curves under both resting and activating conditions (χ2 goodness of fit of 2.8 at pH 7.5 and 2.6 at pH 3.0) (Fig. 3). The second-best match was obtained with the open structure of GLIC, but this only resulted in moderately good fits, with a χ2 of 7.3 under activating conditions and slightly higher (8.8) for the resting-condition measurement. The models based on the ELIC/sTeLIC homologs resulted in clearly worse fits than either GLIC structure, indicating that the room temperature solution-phase structure of GLIC indeed is better described by the GLIC conformations observed by X-ray crystallography than by more open or closed conformations observed in related channels. The wide pore and contracted ECD conformation of sTeLIC was a particularly poor model for solution-phase GLIC (χ2 of 22 at pH 7.5 and 20 at pH 3.0).
GLIC solution scattering corresponds to resting X-ray structures. Fits of model spectra calculated from crystal structures to SANS data collected at (A) pH 7.5 (resting conditions) and (B) pH 3.0 (activating conditions). Based on the goodness of fit (χ2), the individual structure that provided the best fit to both measurements was closed-state GLIC, with the second-best (although substantially worse) match between open-state GLIC and activating condition measurements. Any linear combination of theoretical curves made the fit to resting-state data worse, while up to 18% of the open structure leads to at least equally good fits to the activating conditions SANS curves (SI Appendix, Fig. S1 and Table S2).
Fitting of a linear combination of the theoretical curves from the closed and open models of GLIC revealed that including any fraction of the open model worsened the goodness of fit to the resting-state data, while including more than 18% from the open structure worsened the fit at activating conditions (SI Appendix, Fig. S1 and Table S2).
Since comparisons to individual X-ray structures did not conclusively discriminate differences between resting and activating conditions in solution, we tested whether the data could be better described with sampling of more diverse regions of the conformational landscape. This can in principle be achieved with MD simulations; provided the simulations are started in many different regions of the conformational space they should provide an ensemble of conformations that approximate the flexibility and motions present during gating. To generate such an ensemble, we used coarse-grained elastic network extrapolation between the open and closed structures of GLIC, followed by reintroduction of atoms absent in the coarse-grained extrapolation (side chain reconstruction) and MD simulations from these seeds. After side chain reconstruction, energy minimization, and equilibration, the fits showed major improvements over experimental X-ray structures to a best χ2 of 1.4 to the pH 7.5 data and 1.5 to the pH 3.0 data. This was improved just-so-slightly further after 200 ns of production simulation (best χ2 of 1.3 to the pH 7.5 data and 1.4 to the pH 3.0 data) (SI Appendix, Fig. S2). The best-fitting models had theoretical curves in close agreement with the scattering curve, including in the 0.10–0.12 Å−1 region (Fig. 4 A and B). A summary of the modeling using crystal structures and MD simulations is available in SI Appendix, Table S3.
Comparison of molecular simulation ensembles to experimental SANS data at pH 7.5 and 3.0. (A and B) Experimental SANS curves overlaid with theoretical scattering curves from models after 200 ns of MD simulations. Colors represent goodness of fit to the experimental curve. The magnified Inset corresponds to Q∈[0.1,0.2] Å−1. (C and D) Conformations from simulation ensembles projected onto the two lowest principal components, PC1 and PC2, of the landscape constructed from 46 GLIC crystal structures (blue stars), with the main motion captured by each PC illustrated by the dark half of the cartoons on the axes. The energy minimized interpolated conformations used as simulation seeds are shown as black triangles. Colored data points represent conformations after 200 ns of MD simulations, with shade representing their χ2 goodness of fit to the pH 7.5 (C) and 3.0 (D) experimental SANS data. E and F project all simulation conformations on PC1 along the x axis, while the y axis shows χ2 for data at pH 7.5 (E) and pH 3.0 (F). Finally, G and H display the same data but instead projected onto PC2 at pH 7.5 (G) and pH 3.0 (H).
Principal component analysis (PCA) can be used to identify which motions in a system explain most of the observed variance. Using the first two PCs defined by 46 experimental GLIC structures (34), models that best fit SANS data under resting conditions largely correspond to the lowest coordinates of both PCs, near the closed X-ray structures (Fig. 4C). In contrast, for the SANS data from activating conditions the region with the best-fitting models was shifted toward the open structure (Fig. 4D).
The first PC largely corresponded to the expansion of the ECD, while the second mainly captured the contraction of the upper part of the pore (Movies S1 and S2). By correlating the goodness of fit to each PC, it is possible to assess how these conformational features impact the fit. For the resting-state SANS data, the goodness of fit is clearly best (lowest) at the coordinates corresponding to the closed GLIC structure, and it increases almost linearly as the system undergoes the transformation toward the open state both for PC1 (Fig. 4E) and PC2 (Fig. 4G). In contrast, at activating conditions the best fit is achieved for ensemble simulation states from roughly halfway between closed and open GLIC states (Fig. 4 F and H). Based on the motions the PCs correspond to, this indicates that under resting conditions the best fit is achieved with a fully expanded ECD and a contracted pore, while an intermediate expansion of the ECD yields the best fit to SANS data from activating conditions, with tolerance for a range of pore conformations (Fig. 5).
Conceptual models of GLIC conformational distributions. Under resting conditions, the average solution structure of GLIC is similar to the closed X-ray structure, while activating conditions appear to favor an intermediate ECD-expansion and a range of possible pore conformations, which could also correspond to a mix of multiple discrete conformations.
By comparative fitting of SANS curves to crystal structures and enhanced-sampling MD ensembles, we have demonstrated that it is possible to use SANS to detect quite subtle conformational changes in pentameric ligand-gated ion channels in solution phase at room temperature, and test structural hypotheses. Using crystal structures of GLIC and related pLGICs, we were able to rule out substantial contributions from putative alternative states containing a contracted ECD and either a wide or tightly closed pore, and we identify the closed GLIC X-ray conformation as the best single-structure representative (χ2 ≤ 2.8) of the average solution ensemble at resting conditions, while the data at activating conditions is compatible with a mix of up to 18% contribution from the open structure. By further sampling conformations along the predicted gating transition and using an ensemble of MD simulation structures, we were able to achieve significantly better fits to both resting and activating condition SANS data, and can clearly discriminate between structural models to fit the data. The differences are mainly evident in the shift of the scattering curves at high Q (>0.1 Å−1). This suggests a model for the conformational distribution where the average conformation of GLIC under resting conditions has an expanded ECD and contracted pore, and is well described by the closed X-ray structure (Fig. 5). Upon activation (low pH), the average conformation of the ECD partly contracts toward the open X-ray structure, while the average TMD conformation shows a broader distribution that could also indicate a mix of multiple discrete states.
Given the relatively subtle differences in GLIC scattering under different pH conditions, the observations in the present study would not have been possible without several recent experimental advances. As recently demonstrated for other membrane protein families (26, 28, 30), use of deuterated detergent produced scattering data representing the protein alone, simplifying data analysis by allowing the exclusion of micelle parameters. In cuvette mode, GLIC showed evidence of aggregation even when exchanged by gel filtration on the same day (Fig. 2B). Switching to the SEC-SANS configuration proved to be instrumental in reducing the contribution of apparent aggregates. A drawback of this mode is that continuous SEC-SANS requires the sample to be split over multiple runs in order to measure at multiple detector distances: For GLIC as for many macromolecules, collection at two distances was necessary to obtain both low-resolution information related to sample mass (QRg ≤3) and higher-resolution information related to functional state (Q > 0.1 Å−1) (Fig. 2A). Despite requiring some technical finesse, manual suspension of SEC flow and temporary relocation of the SANS detector during peak elution enabled measurement with minimal aggregates over an extended Q range for a single sample load (Fig. 2B); this “paused-flow” configuration will likely be applicable to other biological macromolecules. With the latest update to the D22 instrument at the Institut Laue–Langevin neutron source, the upgrade to two detector banks will allow measuring the Q-range from 0.003 to 0.7 Å−1 in a single run without manipulating the detector position during the run. Finally, to sample beyond the available crystal structures, coarse-grained interpolation followed by all-atom MD simulations proved highly efficient in modeling GLIC SANS curves (Fig. 4). Given the number of interpolation seeds used, even the modest simulations implemented here (200 ns each) could constitute a prohibitive computational cost (20-μs total simulation time) for similar future work. However, we noted a similar quality of fits for relaxed simulation seeds as for simulation endpoints (SI Appendix, Fig. S2), which suggests the production runs could possibly be much shorter in many cases.
SANS primarily differentiates structural models on the basis of large-scale conformational changes that affect, e.g., the peripheral radius. In GLIC, such transitions primarily involve the contraction or expansion of the ECD. Accordingly, the best-fitting crystal structure at both pH conditions was closed (resting state) GLIC, due to its more expanded ECD (Fig. 3). Indeed, in linear combinations, only a minority of the open GLIC structure was tolerated (SI Appendix, Fig. S1 and Table S2). Notably, lattice interactions in pLGIC crystals primarily involve the ECD periphery, as other regions are embedded in or proximal to the detergent micelle; it is possible that more contracted forms of the ECD might be preferentially stabilized by crystal contacts, while a slightly more expanded state predominates in solution. Still, the best-fit MD-simulation models under resting conditions corresponded to the closed crystal structure not only along PC1, which primarily represented ECD blooming, but also along PC2, representing upper-TMD contraction (Fig. 4). We found no evidence for states comparable to ELIC or sTeLIC in GLIC solution structures. Aside from a contracted ECD, ELIC- and sTeLIC-based models contained tightly closed or wide-open pores, respectively (Fig. 1), which did not appear to contribute based on the distribution of best-fit MD models along PC2; instead, resting conditions corresponded closely to the closed crystal structure, while activating conditions were best modeled by simulation ensemble members roughly halfway between open and closed X-ray structures. The locally closed state of GLIC (8) projects to intermediate values in the PCA (Fig. 4), a bit closer to the open crystal structure than the optimal region for fit to the activating condition SANS data. The locally closed structures have a contracted ECD like the open structure and a closed pore, but the average solution conformation at activating conditions appears to be somewhat closer to the closed crystal structure in both the ECD and TMD. Thus, our results emphasize expansion of the ECD relative to open GLIC crystal structures, but also highlight moderate pH-dependent changes in the TMD.
While the optimized models achieved a good fit at most Q values, there was for all models a systematic difference at low Q. As Q is related to real space distances d by d=2π/Q, the low Q region corresponds to the longest length scales in the system. In the Guinier region—where the distances probed exceed those within the protein—the errors were small so, even slight differences of the model in this region can be expected to have a large impact on the error-weighted residual. For the MD simulation models the deviation at the lowest Q values can in part be explained by a slight underestimation of the molecular weight as the models included only residues corresponding to those resolved in the crystal structures—they thus lacked a few terminal residues compared to the measured protein. In the Q range corresponding to the longest distances within the protein (d≈110 Å, Q ≈0.06 Å−1) the trend was for models with a larger radius of gyration to have a smaller residual, which is consistent with a more expanded conformation of the protein. The model optimization mainly impacted the region containing the main feature in the data (Q ∈[0.10,0.12] Å−1), which corresponds to finding models where internal distances of ∼50–60 Å match those of the probed solution structure. At the largest Q values with experimental data of sufficient quality, corresponding to probed distances between ∼20 and 50 Å, there were minor differences between the models and experimental data, indicating that while the models may be good, they are not perfect at describing this distance range.
Fits to scattering curves under resting conditions (pH 7.5) contrasted with those under activating conditions (pH 3.0). For the latter, SANS was best described by conformations interpolated between resting and open crystal structures. Because SANS measures an ensemble, an intermediate model may represent a distinct dominant state, or the average of a heterogeneous mixture. Indeed, several structural studies have supported the presence of multiple states in equilibrium at low pH. One GLIC variant was crystallized at pH 4 in two partially occupied forms, both with a contracted ECD, but with either a closed or open pore (6). Atomic force microscopy images have also revealed a mixture at low pH, including states that predominated under neutral resting conditions (24). We have also recently used cryo-electron microscopy of GLIC to reconstruct multiple particle classes at pH 3, including a dominant state with an expanded ECD and closed pore, and a minority class intermediate between resting and open crystal structures (7). Due in part to its low conductance in single-channel recordings (35), the open probability of GLIC has not been well established. However, there is precedence in this family for flickering between states even at high agonist concentrations (36, 37) and for open probabilities well below 100% at activating conditions (3841). In recent computational work, we estimated the low-pH open probability of GLIC to 17% using Markov state modeling (42)—notably similar to the tolerated contribution (18%) of the open structure in our linear-combination fits (SI Appendix, Fig. S1 and Table S2). There are thus several indications the SANS curve under activating conditions might be due to an average of mixed states, mostly closed, but some similar to open crystal structures. Prolonged activation leads to desensitization in GLIC (43, 44), so pH 3 conditions should also be expected to produce a population of desensitized channels, distinct from both resting and open crystal structures. It has been proposed that desensitization mimics the lipid-modulated crystal structure of GLIC, with a contracted ECD and partially expanded pore (11), slightly different from the intermediate ECD contraction of the average pH 3 structure. Contribution from this or another desensitized form, either within or beyond regions sampled in our MD simulations, may prove a productive area of future investigation.
In conclusion, paused-flow SEC-SANS proved effective in determining distinct room temperature solution structures of a key model pentameric ligand-gated ion channel under resting versus activating conditions. These solution structures, deposited in the Small-Angle Scattering Biological Database (SASBDB) (45), should prove valuable as reference points for the average conformation of GLIC in solution, and as models that can be used in future studies, e.g., as launching points for MD simulations. Furthermore, our results from pH 3 represent an independent experimental technique pointing to a closed majority population under activating as well as resting conditions. Additionally, we have demonstrated a readily applicable enhanced sampling approach for generating physically plausible models, which can be used to fit small-angle scattering data. The resolution of this SANS approach is still limited by the signal-to-noise ratio at high Q in the experiment. However, this is expected to improve by an order-of-magnitude as next-generation neutron setups (e.g., the European Spallation Source) become available in coming years, which will enable lower sample volumes and correspondingly higher concentrations. This should make it a very powerful method for discerning even small conformational transitions of biological macromolecules under realistic solution-phase and room temperature conditions.
GLIC was expressed and purified as previously described (5, 46). In short, a fusion protein of GLIC and maltose binding protein (MBP) in a pET-20b–derived vector was expressed in C43(DE3) Escherichia coli cells. Cells were inoculated 1:100 into 2xYT media with 100 μg/mL ampicillin, grown at 37 °C to a OD600 of 0.7, at which time they were induced with 100 μM isopropyl-β-d-1-thiogalactopyranoside, and shaken overnight at 20 °C. Cells were harvested through centrifugation and lysed by sonication in buffer A (300 mM NaCl, 20 mM Tris⋅HCl, pH 7.4) supplemented with 1 mg/mL lysozyme, 20 μg/mL DNase I, 5 mM MgCl2, and protease inhibitors. Membranes were harvested by ultracentrifugation and solubilized in 2% n-dodecyl-β-d-maltoside (DDM), following which the GLIC-MBP fusion protein was isolated through amylose affinity purification and size exclusion chromatography in buffer B (buffer A with 0.02% DDM). The fused MBP was cleaved from GLIC using thrombin, and an additional size exclusion chromatography yielded pure GLIC in a buffer B.
SANS experiments (47, 48) were performed at the D22 beamline of Institute Laue–Langevin, using three experimental setups: cuvette-mode SANS, continuous-flow SEC-SANS, and paused-flow SEC-SANS (see SI Appendix, Tables S4 and S5 for detailed sample and collection parameters). The scattered intensity I was measured as a function of the momentum transfer Q—which is given by Q = (4π/λ)sin(θ), where 2θ is the scattering angle and λ is the wavelength.
Cuvette mode allowed the full amount of sample to be measured for both detector distances (2m/2.8m, and 11.2m/11.2m) required to achieve the full Q range; it did not, however, ensure sample monodispersity. To measure monodisperse samples, SEC-SANS (31, 32) was used, running a gel filtration with a Superdex 200 Increase 10/300 column in-line with the SANS measurement. In the continuous-flow measurement, the sample was divided over two runs, one for each detector setting (2m/2.8m, and 11.2m/11.2m), and the flow speed decreased to 0.05 mL/min upon peak detection by UV-vis absorbance at 280 nm. In the paused-flow measurements, the full sample amount was loaded for a single run; the flow was slowed down to 0.01 mL/min upon peak detection and paused at the peak max to enable the second detector distance measurement (2.8m/2.8m during the run, and 8m/8m while paused). The gel filtration step was used to exchange the sample to D2O buffers with match-out deuterated DDM (d-DDM) (26). For the cuvette-mode experiment, this was performed off-line to the SANS experiment and the exchanged sample loaded into 2-mm Hellma quartz cuvettes. All three experimental setups were used to measure GLIC at pH 7.5 (150 mM NaCl, 20 mM Tris⋅HCl, pH 7.5, 0.5 mM d-DDM); GLIC at pH 3.0 (150 mM NaCl, 20 mM citrate⋅HCl, pH 3, 0.5 mM d-DDM) was measured using the paused-flow setup.
Data reduction and buffer subtraction were performed using Grasp, version 9.04 (49), utilizing either detector frames preceding the protein peak (standard SEC-SANS) or dedicated buffer measurements for the buffer background (paused-flow SEC-SANS and cuvette SANS). Data were corrected for the empty cuvette and background, and scaled by their transmission and thickness. They were scaled to absolute intensity by direct flux measurement. Concentration normalization of the paused-flow SEC-SANS runs were performed by dividing the scattering intensity with the concentration calculated using the absorbance at 280 nm from the corecorded chromatograms and the extinction coefficient calculated from the amino acid sequence using ProtParam (50). In merging data from the two detector distances, data from the detector distance covering low Q (11.2 m for continuous-flow and cuvette-mode, 8 m for paused-flow) were used up to 0.070 Å−1 continuous-flow and cuvette-mode, and up to 0.095 Å−1 for paused-flow. Above this, data from 2 m (continuous-flow and cuvette-mode) and 2.8 m (paused-flow) were used. A small additional constant was subtracted as a final adjustment to the background.
Models based on X-ray crystal structures were constructed using PDB entries 4NPQ (GLIC, closed conformation) (6), 4HFI (GLIC, open conformation) (51), 3RQU (ELIC) (52), and 6FL9 (sTeLIC) (22). Using Modeler (53), terminal residues to match the construct used in the SANS experiment were introduced to the models based on 4NPQ and 4HFI, as were residues missing in the 4NPQ structure. For the models based on 3RQU and 6FL9, homology models of GLIC with these structures as templates were constructed using Modeler. The N-terminal residues that were present in the construct but not in the crystal structures were visually inspected in the models, and a single conformation of these residues was chosen and introduced to all subunits in all models.
Models based on MD simulations were obtained from simulations started from seeds along an open-closed transition pathway generated from coarse-grained elastic network-driven Brownian dynamics (eBDIMS) (34, 54). Two transition pathways were constructed between X-ray structures 4NPQ and 4HFI resulting in a total of 50 seeds (25 for each pathway using either 4HFI or 4NPQ as eBDIMS target). The atomistic detail was then reconstructed using Modeler (53) in two steps; first, side-chain conformations were transferred from 4HFI and energy minimized to fit the new conformation, followed by refinement of hydrogen bonds to ensure all secondary structure elements remained intact. Each initial conformation was then protonated to mimic both activating or resting conditions, resulting in a total of 100 initial conformations—50 at activating and another 50 at resting conditions. For activating conditions, a subset of acidic residues were protonated (E26, E35, E67, E75, E82, D86, D88, E177, E243; H277 doubly protonated) to approximate the predicted pattern at pH 4.6, as previously described (55). Conformations were embedded in a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine bilayer, solubilized with TIP3P water and 0.1 M NaCl, energy minimized, and equilibrated for 76 ns with gradual release of restraints. From the equilibrated systems, unrestrained MD simulations were launched, and frames after 200 ns of simulation time were extracted and used as models to fit against the SANS data. Terminal residues present in the experimental construct but not in the crystal structures were omitted in the simulations and extracted models. Energy minimization, equilibration, and MD simulations were performed using Gromacs (56), versions 2018.4 and 2019.3.
Guinier analysis was performed to calculate I(0) and radius of gyration (Rg). In the Q range from 0.010 to 0.046 Å−1 (approximately QRg = 3) ln(I) was plotted as a function of Q2, to which a line was fitted. By applying the Guinier equation, the fitted line yielded I(0) from the intercept, and Rg from the slope. With I(0) determined, the molecular weight was calculated using the following:Mw=NA⋅I(0)c(Δρ⋅ν¯)2,[1]where NA is Avogadro’s constant, c is the protein concentration, Δρ is the excess scattering length density, and ν¯ is the partial specific volume. The excess scattering length density is given by |ρproteinρbuffer|, which was calculated using the scattering length density for GLIC and for D2O (SI Appendix, Table S3). For GLIC, the hydrogen–deuterium exchange of labile hydrogens was taken into account by calculating the expected degree of exchange after 100 min (approximately the time in the paused-flow experiments from the start of a run until the peak max) at pH 7.5 and pH 3.0 using PSX (protein–solvent exchange) (57) and the model based on the closed GLIC structure. To account for GLIC being a transmembrane protein, which means that labile hydrogens in the transmembrane region are shielded to a larger extent than in a soluble protein, hydrogens within the transmembrane region as defined when submitting the model to the Positioning of Proteins in Membranes (PPM) webserver (58) were considered as 1H. The partial specific volume was estimated by dividing the volume of the closed pore model, calculated using 3V (59), by the molecular weight expected from the amino acid sequence. The pair distance distribution was calculated from experimental curves using BayesApp (60)—available at https://somo.chem.utk.edu/bayesapp/—and for the closed GLIC model using CaPP (calculating pair distance distribution functions for proteins) (61). Theoretical curves and fits to the experimental curves were calculated using Pepsi-SANS (62). Linear combinations of theoretical curves (following the expression Icombination = k⋅Imodel A + (1 − k)⋅Imodel B, where I is intensity and k is the contribution from the first model) were tested using the curves calculated for the models of closed and open GLIC. The goodness of fit of the linear combinations to the experimental data were evaluated by calculating the reduced χ2, approximating the degrees of freedom with the number of data points.
PCA was performed using MDanalysis (63, 64), first constructing a PCA landscape using Cartesian coordinates from the set of 46 GLIC structures used by Orellana et al. (34). The models from MD simulations were projected onto this landscape and the correlation between χ2 and the first two PCs evaluated.
Graphs were plotted using Matplotlib (65), and protein images were rendered using Vmd (66). A summary of software and equations used is available in SI Appendix, Table S6.
The raw experimental data have been deposited at https://doi.org/10.17605/OSF.IO/TDZX4 for cuvette-SANS and continuous-flow SEC-SANS (47, 67), and at https://doi.ill.fr/10.5291/ILL-DATA.8-03-1002 for paused-flow SEC-SANS (48). Processed SANS data and representative models have been deposited in the Small-Angle Scattering Biological Data Bank (SASBDB), https://www.sasbdb.org/project/1317/ (paused-flow SEC-SANS at pH 7.5 [SASDL33], paused-flow SEC-SANS at pH 3 [SASDL43], continuous-flow SEC-SANS at pH 7.5 [SASDL53], and cuvette-SANS at pH 7.5 [SASDL63]) (68).
We acknowledge the Institut Laue–Langevin and European Synchrotron Radiation Facility for developments in SEC-SANS and allocated beam time at the D22 instrument. This work was supported by grants from SwedNess, the Knut and Alice Wallenberg Foundation, Swedish Research Council (2017-04641, 2018-06479, and 2019-02433), Swedish e-Science Research Centre, BioExcel Center of Excellence (EU 823830), and the Lundbeck Foundation through the Brainstruc grant. Computational resources were provided by the Swedish National Infrastructure for Computing.
Author contributions: M.L., L.A., R.J.H., and E.L. designed research; M.L., U.R., C.B., N.T.J., A.M., L.P., and R.J.H. performed research; M.L., U.R., C.B., N.T.J., A.M., L.P., L.A., R.J.H, and E.L. analyzed data; and M.L., U.R., C.B., N.T.J., A.M., L.P., L.A., R.J.H., and E.L. wrote the paper.
The authors declare no competing interest.
This article is a PNAS Direct Submission.
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Start Stopping Faster – Harvard Business Review

A perennial executive challenge is stopping mediocre to failing businesses and projects sooner rather than later. But only by doing so can they free up the resources needed to pursue more opportunities. Three ways to get better at killing things sooner is to make decisions reversible (e.g., by conducting more experiments), make work more visible to everyone so it’s easier to see what work is being done and how well it’s going, and overpower the fear of failure and not having a job if someone’s current project is killed.
Business executives could learn a lot from cheetahs, Earth’s most agile land animals. Though their ancestors ran only about 20 miles per hour, today’s cats can accelerate from zero to 60 within three seconds — faster than a Corvette Twin Turbo or a Ferrari Enzo. But speed alone is not what makes cheetahs such awesome hunters. Computer models show that the best predictor of a successful hunt is not a cheetah’s top speed; rather, it’s how fast it stops and turns.
There is an important parallel to the executive hunt for innovations. Whether they are developing new products, processes, or overhauling old ways of doing business, it’s not enough that organizations pursue new ideas faster. Unless they develop new muscles for skillfully decelerating and adapting to unexpected twists and turns, they are likely to come up empty-handed. It’s one of the most common laments of executives struggling to increase their organization’s adaptability: “We are terrible at stopping work, even when it’s obvious that the work is a complete waste of time and money.” This is as true for existing business lines and processes that live on budget season after budget season zombie-like as it is for a once-bright new idea that simply isn’t panning out.
The cost of this problem is higher than managers imagine. Gary Hamel and Michele Zanini estimate the cost of bureaucratic waste has hit $10 trillion and is growing. Between 70% and 90% of innovations fail, and healthy operations grow weaker every day that they must subsidize foundering projects kept alive by political inertia rather than potential payoff. When power is determined by the amount of resources controlled, as it so often is in business, admitting failure and surrendering resources is rare.
Since stopping things is so very hard, executives make starting them even harder, dampening innovation. They raise investment hurdle rates, demand more detailed analyses, and add layers of scrutiny. Sadly, these actions don’t improve decisions so much as damage speed to market and competitive positioning. Failures mount. Eventually, the horde of failing projects grows too large to ignore. Managers cull some large percentage of their people, traumatize the organization, and launch the doom loop all over again.
There is another way. Organizations can evolve and by focusing on three specific things, they can improve their own agility and start stopping things faster.
While researching our book Doing Agile Right: Transformation Without Chaos, we learned from former Amazon executive Jason Goldberger that in order to accelerate innovation, founder Jeff Bezos purposefully encourages executives to make decisions reversible, which ensures that a company won’t have to live with bad consequences for very long. It thwarts risk aversion and accelerates experimentation.
“If you tell people to innovate without making mistakes, you will kill innovation,” Goldberger explains. “But if you tell people to innovate and not worry about mistakes that are quickly reversible, you free them to test and learn in more agile ways.”
Unfortunately, not many companies run this way. Far too many investment proposals plan for premature and irreversible scaling. They call for large upfront investments, and predict delayed, hockey-stick-shaped revenues and profits. When revenue and profit fail to materialize, it seems too late to stop. The payoff must be just around the corner. “Why, we would be crazy to stop now,” executives tell themselves. And so, throwing good money after bad drags on.
One way to break this habit is to run the business the way a savvy venture capitalist invests. Recognize business plans for what they really are: business experiments. Break large, risky gambles into a series of smaller, smarter tests. Clarify the hypotheses, the best ways to test them, and the metrics that will signal whether to persist, pivot, or pause. Avoid premature scaling — hiring too many people, building too much capacity, doing too much marketing — before key assumptions have been validated. Match costs to revenues. Start by confining experiments to affordable, adaptable, and reversible microcosms of the ultimate solution by limiting geographies, customer segments, or product lines. (You can read more on how to experiment effectively, in this HBR article by Stefan Thomke and Jim Manzi.)
Look at DoorDash, the door-to-door delivery company. Recently DoorDash has ridden a Covid-19 surge in demand, but back in 2013, when it began raising what would eventually become a total of $2.5 billion, venture capitalists didn’t just back up a Brink’s truck of money to the startup. The risks were too high, and such a move would have limited investors’ ability to spur strategic change when necessary. Instead, venture capitalists phased in their investment over 11 rounds of funding.
In the 2016 round, when questions about the viability of its strategy reportedly led to its shares selling at a lower price than earlier rounds, the company made important changes. DoorDash added new services for restaurants and adjusted pay for drivers. Market share increased, as have subsequent valuations.
DoorDash is still not profitable, and its ultimate success is far from guaranteed. But its venture backers have clear and frequent opportunities to change how they invest and influence company direction. Investors can decelerate, pivot, and stop.
Some corporations are already applying this model. Executives review new projects and existing business lines quarterly, utilize fast feedback loops, create rough prototypes, and rely on objective metrics to test key hypotheses. All this makes it possible to more dynamically adjust plans and allocate resources.
It’s hard to improve or stop unproductive work if you can’t see what work is being done and how well it’s going. Too many companies are flying blind.
Executives can inspect physical facilities and assess whether to refurbish or bulldoze them. They can see inventories piling up and decide whether to finish them or write them off. The intangible work of most technology, marketing, and other departments, however, is often invisible to leadership teams.
Increasing visibility is good for everyone. It helps senior executives uncover valuable initiatives, recognize the people pushing them, and accelerate their progress. It allows employees to see projects related to their own jobs, learn from them, and identify where their expertise could solve perplexing problems or save time and money. It makes it easier for everyone to identify duplicative work and triggers discussions about whether overlapping teams should collaborate or compete. It helps teams working on interdependent steps coordinate and minimize delays.
Imagine a system that enables authorized employees to see all work streams, who is on each team, what else they are working on, and how the work is progressing. Imagine tagging the work of each team with descriptors such as strategic priority, targeted customers, expected economic value, and progress against plans. Perhaps employees could even express how confident they are in each team’s success. These systems actually exist — project and portfolio management software, objectives and key results trackers, talent management systems, and workforce analytics — and they are getting better.
They say the first rule of daredevil airplane wing-walking is: “Don’t let go of what you’ve got until you get hold of something better.” Astute leaders realize that fearful workers will cling to the current work no matter how unproductive. They do several things to overcome that fear. One we’ve already discussed:  They reduce the cost of stopping projects (e.g., by conducting experiments).
Another way is to reward people who learn valuable lessons by taking prudent risks, even if the immediate outcome was disappointing. In some cases, this may mean keeping the bold objective but adapting the approach as conditions or capabilities change. When Bezos wanted to enable third-party sellers to sell new or used products on Amazon, the company initially failed: The 1999 launches of Amazon Auctions and zShops both fizzled. But Amazon Marketplace, launched soon after, was a success. It now accounts for more than half of all units sold by the company.
Finally, giving people more opportunities if their current project fails reduces the likelihood that they’ll stick with a bad idea longer than they should. Successful companies build a strong and visible backlog of compelling opportunities. They make it clear that until existing projects that aren’t panning out have stopped, new initiatives can’t be launched. And they redeploy people from the former to the latter as a matter of policy and offer training to ease the transition. In time, the fear of missing out on something better starts to overpower the fear of loss.
In a world of increasingly unpredictable change — where opportunities are constantly zigging and zagging like spry gazelles — running at higher speeds is not enough. Businesses must evolve to match their acceleration muscle with faster stopping and turning skills. As they do, their hunt for growth will grow more fruitful, their competitive capabilities will strengthen, and their position in the food chain will climb.

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The Samsung Galaxy Z Flip 3 Is Finally Here—Check Out The Best Places To Buy One Today – Forbes

The Samsung Galaxy Z Flip 3 is headed to doorsteps August 27, and there are plenty of reasons to … [+] consider pre-ordering.
If you’re looking to upgrade your smartphone and love the look and function of Samsung’s latest foldables, the new Galaxy Z Flip 3 5G has just been released, so now’s the perfect time to get your hands on what has become an instantly popular smartphone. There are awesome deals to cash in on if you decide to buy, whether you get it from Samsung, Best Buy, AT&T or another popular service carrier. 
While you can purchase the phone outright (MSRP: $999.99 for the 128GB model), most of the best deals are offered to people who trade an existing phone and then finance the new phone for up to 36 months. 
Samsung is offering up to $600 off on the purchase of the phone with an eligible trade-in, bringing the price down to $399.99 for the 128GB model. In conjunction with this deal, the phone can be financed for $11.11/month for 36 months, and a free silicone cover (up to a $39.99 value) is included.
Samsung
Purchase the Galaxy Z Flip 3 5G directly from Samsung and you’ll also receive three months of Spotify Premium, four free months of YouTube Premium and six free months of SiriusXM streaming.
Hop on over to Best Buy to pre-order the Galaxy Z Flip 3 5G and get $400 in instant savings with activation (even for an unlocked phone), and get up to 24 months financing. An additional $200 in Samsung Credit is also being offered to qualifying new and existing customers.
Best Buy
Alternatively, when pre-ordering and activating the phone from Best Buy—with AT&T, T-Mobile, or Verizon as your chosen service provider—a savings of up to $400 is being offered with qualified activation. This goes along with an additional $1,000 credit for an eligible trade-in, and up to a $200 Samsung Credit that can be used toward accessories or a memory upgrade for the phone.
When you purchase the phone through Best Buy and then activate it with a particular service provider, alternate deals are also available based on your current service contract or whether the phone will be activated to a new account.
AT&T is offering up to $1,000 in bill credits, plus up to $350 more in additional bill credits when you buy on a qualifying installment plan with an eligible AT&T unlimited plan (minimum $75/mo. before discounts) and trade in an eligible smartphone. Additional restrictions may apply.
Without a trade-in, the phone can be financed for $27.78/month with 0% APR for 36 month. If you sign up for the AT&T Next Up offer for an early trade-in after paying off 50 percent of the phone, you can take $5/month off the monthly bill.
AT&T
AARP members can also save up to $10/month per phone line on an AT&T Unlimited Elite plan and pay no activation or upgrade fees with select upgrades.
T-Mobile is offering a “Get One Free” deal when you buy the Galaxy Z Flip3 5G and take advantage of the company’s 24-month financing option, activate a line on an eligible service plan, and trade-in an eligible device.
US Cellular is offering up to $1200 off any 5G smartphone, including the Galaxy Z Flip3 5G, which makes the phone free when you sign up for an Unlimited Even Better service plan, with credit approval, and commit to a 30-month service plan. An additional trade-in credit for an eligible phone may also be available. 
Samsung improved the durability of the new phone model, tossed in a better processor, and redesigned the Cover Screen. Last year’s Galaxy Z Flip model showcased what a foldable phone for the masses would look like. And despite the Z Fold2 gaining more headlines, the Flip 5G was a great option if you didn’t want to carry a tablet in your pocket.
Samsung Galaxy Z Flip 3 on table
Fast forward to today, and the Galaxy Z Flip3 5G is here with plenty of intriguing upgrades and seven casing colors to choose from, including cream, black, green, lavender, gray, white, and pink. Choose between 128GB and 256GB of internal storage.
With the various upgrade and trade-in deals being offered, the 256GB model (MSRP: $1,049.99) becomes extremely affordable. Of course, the new Galaxy Z Flip3 5G is a 5G-compatible smartphone, so right out of the box, you can expect the fastest cellular data connection speeds your service provider allows.
Samsung
Price: $999 | Rear Cameras: 12MP wide, 12MP ultra wide | Selfie Camera: 10MP | Processor: Qualcomm Snapdragon 888 (5 nm) | Main Display: 6.7-inch Dynamic AMOLED 2X Infinity Flex, 120Hz, 1080 x 2640 pixels, 425 pixels per inch | Cover Screen: 1.9-inch Super AMOLED, 260 x 512, 302 pixels per inch | Storage: No microSD slot, 128GB or 256GB internal | Memory: 8GB | Battery size: 3,300 mAh | Dimensions: 72.2 x 86.4 x 17.1 mm (Folded), 72.2 x 166.0 x 6.9 mm (Unfolded) / 6.54” x 2.84” x 0.27” (folded), 3.40” x 2.84” x 0.67-0.32” (Unfolded) | Weight: 183g (6.46 oz.)
Under the hood, there really aren’t too many differences between the Galaxy Z Flip 5G and the Galaxy Z Flip3 5G. Those wondering about how Samsung arrived at the Z Flip3 will simply have to look at the history of this clamshell. Technically, this is the third clamshell folding phone to be released, and Samsung wanted to provide some uniformity in its new Galaxy Z lineup.
Different Samsung Galaxy Z Flip 3 Color options
While the main screen of the Z Flip3 5G is the same size as its predecessor; coming in at 6.7-inches, there are a few notable improvements. First is the upgraded display technology being used. It’s also a Dynamic AMOLED display, which Samsung claims has been improved to the point where it’s 80% more durable than last year’s phone.
Even with the improved display, the phone’s battery boasts up to 25 hours of talk time, or up to 9 days and 4 hours of standby time.
Keeping with durability for a moment, the Z Flip3 5G marks the second folding phone to sport an official IP rating. Thanks to the IPX8 water resistance, the Z Flip3 5G can be submerged in 1.5 meters of water for about 30 minutes. We don’t recommend putting those claims to the test, but at least the phone will survive being stuck in a rain storm.
Samsung Galaxy Z Flip 3 Water Resistance Promo
Additionally, the Z Flip3 5G offers an upgraded refresh rate with its 120Hz display, up from the 60Hz refresh rate from the Z Flip 5G. This matches up with what we have on the Galaxy Z Fold3, along with many other flagship Android phones that have been released thus far in 2021.
When you have the Z Flip3 5G in clamshell mode, the Cover Screen looks a bit different. Samsung has integrated both the Cover Screen and dual-camera system into the slab of glass that stretches the width of the phone. The Z Flip3 5G now features a 1.9-inch Super AMOLED cover display, making it better to use when taking selfies or managing your incoming notifications.
As for those aforementioned cameras, you’ll notice that there’s really not much of a difference from the Z Flip 5G. There’s a dual-camera system with a 12MP wide-angle main camera, along with a 12MP ultra-wide sensor placed in a vertical orientation.
If you look at the Android flagship phone landscape, just about every option is powered by the latest Snadpragon 888 chipset. This includes the Galaxy Z Fold3 5G, and Samsung is bringing the same processor to the Z Flip3 5G. With this update, there’s about a 25% difference in single-core performance along with 12% increase in multi-core performance.
Samsung is keeping the same 8GB of RAM from the Z Flip 5G, but instead is offering either 128GB or 256GB of storage. Adding an additional storage option definitely helped push the price a bit lower compared to last year’s clamshell Galaxy phone.
Samsung Galaxy Z Flip 3 with Galaxy Buds 2 and Galaxy Watch 4
The most exciting aspect of the Galaxy Z Flip3 5G might not just be the new and improved durability or the larger cover screen. The price tag is also much more attractive. The Galaxy Z Flip 5G was released with a launch price of around $1,449. Since then, Samsung has permanently discounted its clamshell phone by 20%, bringing the price down to $1,199.
The new Galaxy Z Flip3 5G and its IPX8 water resistance rating along with its 120Hz refresh rate, is coming in at just $999. Offering a $450 price discount between generations shows Samsung heard the previous complaints loud and clear.
Optional Samsung accessories for the Galaxy Z Flip 3 5G include the Wireless Charger Pad Trio ($89.99), the Wireless Charger Stand 15W ($79.99), the Samsung Wireless Charger Duo ($59.99) and a selection of cases.
Amazon
Amazon
The Samsung Galaxy Buds 2 make an excellent addition to the Galaxy Z Flip 3, allowing for hands-free phone communication and the ability to stream or listen to audio (music, podcasts, etc.) without any headphone cables.
Samsung
Until now, we have not seen a foldable smartphone released under the $1,000 mark. Despite there not being too much competition in the space just yet, perhaps this new (lower) price and compact design will drive folding phones into the mainstream. And maybe that will drive other phone makers to come in with other options in an effort to drop the price even lower.
Between myriad color options and the low starting price, before even considering discounts and trade-ins, the Galaxy Z Flip 3 is already showing signs of being a smash hit.

I am an accomplished author, journalist, and photographer who specialized in consumer technologies.

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Scientists Report Mass Migratory Bird Deaths in U.S. and Mexico – Yale Environment 360


 / 
 
E360 Digest
September 17, 2020
Some of the dead birds found by biologists from New Mexico State University. Allison Salas/New Mexico State University
Scientists and birdwatchers are reporting a mass die-off of migratory birds across the southwestern United States and northern Mexico, several news outlets reported. From flycatchers to swallows to warblers, researchers estimate hundreds of thousands to possibly up to a million birds have died in recent weeks. Scientists say they are unsure of the exact reasons for the deaths.
Avian ecologist Martha Desmond, of New Mexico State University in Las Cruces, called the die-off “unprecedented.”
“It’s enormous, the extent of this,” Desmond told Audubon. “We haven’t counted all the species yet, but there are lots of species involved.”
Online reports show mass deaths in New Mexico, Colorado, Texas, Arizona, and as far north as Nebraska, as well as in four states in Mexico, and include migratory species as varied as owls, hummingbirds, loons, and woodpeckers, many of which were migrating south to wintering grounds.
Many of the dead birds have little fat reserves or muscle mass remaining, and some seem to have literally dropped from the sky mid-flight. Scientists recorded the first group of deaths on August 20 in White Sands Missile Range in New Mexico. Researchers suspect that months of extremely dry conditions, combined with unseasonably cold weather in the Southwest, could have triggered an early start to this year’s migrations before some species were physically ready to make the arduous trip thousands of miles south. But now, as the deaths have continued, researchers say smoke from the ongoing wildfires in California, Oregon, and Washington could also be playing a role.
“It could be a combination of things. It could be something that’s still completely unknown to us,” Allison Salas, a graduate student at New Mexico State University, told The Guardian. “The fact that we’re finding hundreds of these birds dying, just kind of falling out of the sky is extremely alarming … The volume of carcasses that we have found has literally given me chills.”
The dead birds are being analyzed by scientists at the U.S. Fish and Wildlife Service forensics lab in Oregon and the National Wildlife Health Center in Wisconsin. Researchers are urging birdwatchers and volunteers to log sightings of dead birds on the website iNaturalist and contact state Fish and Game agencies or the USFWS.
By Michelle Nijhuis

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Chick-fil-A in Battle Creek Still a Few Months Away – wbckfm.com

The prospect of a new Chick-fil-A restaurant in Battle Creek has lots of people excited, but we’ll just have to be patient for now.   Work started recently to get the lot prepared, where the Ruby Tuesday restaurant once stood.   That building was demolished last November.
A Chick-fil-A, Inc. spokesman tells us, “It’s our pleasure to confirm we will be opening our first Chick-fil-A restaurant in Battle Creek in early 2022.”
The trick now will be to get the lot paved before the asphalt plants close down when the cold weather comes.   And the lot surrounding the new place will be very important, as Chick-fil-A brings a first to the area.
“The new restaurant will feature an innovative dual drive-thru design around the building, which will be the first of its kind in western Michigan”, said a company spokesperson.
This week, Chick-Fil-A opened a new restaurant in South Bend, Indiana, and the company says there’s plenty of other news in the works for our state.  “Chick-fil-A is constantly evaluating potential new locations for expansion. Battle Creek represents a tremendous opportunity for us to serve our customers great food with exceptional care. Additionally, we are planning to open seven new locations in Michigan by the end of 2022.”
As the nearby Lakeview Square Mall struggles to fill its business space,  the Harper Village Shopping Center has added a new Bath & Body Works  and is about to open a new Aldi Grocery Store.  Discount Tire on East Columbia Avenue in Battle Creek has closed and is sending all of its Battle Creek customers to the newer store at Harper Village.
Once Chick-fil-A opens, the entire complex will be at 100% occupancy.  Now we’re wondering how bad the traffic situation will be there.   It’s long been a favorite thing to complain about in Battle Creek.   Will there be improvements?
 
 

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Chrysler's TorqueFlite 727 Transmission Was The Best Muscle Car Automatic Ever Built – Driving Line

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Chrysler was famous for building torque-monster V8 engines during the golden age of the muscle car, with the 440, the 413 Wedge, and the 426 Hemi all stealing more than their share of the spotlight. Less celebrated is the transmission that backed up each of these high performance engines and ensured that all of that hairy horsepower made it to the ground without detonating the driveline along the way, especially as drag racers began to transition from fragile manual boxes to tougher, and more consistent, automatics.
Dodge Demon with 727
The Chrysler TorqueFlite A-727 (better known as just the ‘727’) is to this day one of the strongest automatic transmissions ever built. With a solid reputation for reliability and a proven track record of being able to hold more power than ever left the factory, the TorqueFlite has become not just a ’60s and ’70s icon, but also a sought-after options among project car builders unafraid to mix-and-match across brands.
The 727 appeared in 1962, and it was the second transmission to wear the TorqueFlite name (after the A466). The unit featured an aluminum case versus the iron case of its predecessor, dropping significant weight (with a total mass of 160 lbs), and it offered three forward gears along with a parking lock pawl that had been absent from the A466.
Chrysler 727 Torqueflite transmission cutaway
Much of what helped make the Chrysler 727 so strong had to do with its simple design. All versions featured the same gear ratios: 2.54:1 (1st), 1.45:1 (2nd), 1.01 (3rd) and 2.21:1 (reverse), along with two transmission bands. The transmission was based around a licensed version of the Simpson gear set that also formed the basis for GM’s TH400 and Ford’s C6 automatics. It was also one of the first automatics to incorporate an over-running clutch with a Simpson gear set, and its clutch pack design greatly contributed to its overall strength. Hemi and 440 Six Pack muscle cars featured the beefiest clutch components of all in comparison to base model 727s.
Chrysler 727 Torqueflite transmission cutaway blow-up
During the first few years of its production it went through several updates, moving from a push-button shifter to a cable and then a rod shift linkage, swapping from a canister fluid filter to a Dacron filter, and finally a splined output shaft in place of the earlier flange and universal joint design.
Dodge pickup on Nitto INVO tires
By 1966 Chrysler had fully developed the TorqueFlite and set the design in stone. Only small changes were made from that point on, with a tweaked flex plate added in 1968, a wider front clutch retainer bushing was incorporated in 1971, a larger filter installed in 1973, and a mechanical lock-up torque converter included from 1978-onwards. During the 1980s, the transmission was renamed the 36RH and the 37RH, internal designations that didn’t change the design or execution of how the gearbox was built (although AMC, Jeep, and small block and big block versions of the transmission can feature different bellhousing bolt patterns). 
Mopar produced the 727 TorqueFlite all the way until 1991, when they were last used on the Grand Wagoneer. These transmissions were found in nearly every rear-wheel or four-wheel drive Dodge, Plymouth, or Chrysler model built with a V8 during its 30 years on the market, including a wide range of trucks alongside passenger and muscle cars.
Jeep Grand Wagoneer on Nitto Trail Grappler tires
Today, there are a number of aftermarket companies that manufacture adaptors that allow the 727 to be bolted to the small block Chevrolet V8, a long list of straight six and V6 engines, as well as older Chrysler, Dodge, and Plymouth models that pre-date its 1962 manufacture. The best news for those seeking to install a TorqueFlite 727 in their project vehicle is that modern design has eliminated the need for the stronger Hemi and Six Pack internals, as existing friction materials are much stronger than what was available to Mopar engineers 60 years ago.
Dodge Charger on Nittos
This, combined with an enormous selection of additional upgrades and rebuild parts, means that affordable, easily-found 727s can be inexpensively built to deal with precisely the amount of horsepower their owners plan to throw their way.
Plymouth 'Cuda with 727 automatic transmission
Virtually indestructible when properly maintained or modified, easy to find thanks to their proliferation across Mopar cars, trucks, and SUVs, and simple enough that even novice transmission builders can approach them as a learning tool, the TorqueFlite 727 continues to make waves on the high performance scene nearly three decades after it was taken off the order sheet.
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James Hollingshead Decides to Sit Out 2020 Mr. Olympia – BarBend

 
Eight days ago, on Nov. 4, 2020, James Hollingshead made noise in the bodybuilding world by being the first man to qualify for the 2021 Mr. Olympia. Now, he’s making headlines again by announcing that he will forgo competing at this year’s Olympia. However, he is not the first competitor to drop out. Flex Lewis and Cedric McMillan have already announced that they won’t be competing at the 2020 O, which is set to take place on Dec. 17-20, 2020, in Las Vegas, NV. 
“The Shed” made his intentions known in a video on his YouTube channel, posted on Nov. 12, 2020. It was a decision he credits making with his prep coach, Patrick Tuor. You can check out the video below. 
[Related: The Real Pros and Cons of the Bodybuilding Lifestyle]
“I’m in this place where I sat down, took time to consider all things, and I’m going to be real with you,” Hollingshead said in the video. “After much discussion with Patrick (Tuor), the plan now is to focus solely on improvements, and to shoot for the 2021 Mr. Olympia solely.”
Hollingshead earned the qualification for the 2020 Olympia by winning the Europa Pro in Spain in October of 2020. He flexed his guns and out-muscled Lukas Osladil, who is also qualified for the 2020 Mr. Olympia, for first place. Hollingshead went on to say that competing in the 2020 contest would mean leaving the United Kingdom early due to an impending national shutdown caused by the COVID-19 pandemic. However, he also expressed optimism when talking about his plans.
 
 
A post shared by James “The Shed” Hollingshead (@hollingshead89)

[Related: Hypertrophy Training Sets and Reps]
“For the first time, I can truly concentrate on the bigger picture — the main goal, the big dream,” Hollingshead wrote in an Instagram post. “I suppose all I want to say is that this next show is the one — the big one, the supershow. And we got our pass. Between now and then, we will move mountains to become the best we can be for those that have been behind me and those who are now. It’s going to be Epic.”
In that same post, Hollingshead explains how he’s competed in bodybuilding show after show. To stay in peak condition, bodybuilders need to scrupulously count their macros and stick to a strict and rigorous training split. It’s nearly impossible to gain quality muscle when one is in a constant state of dieting. So now, Hollingshead will have the chance to prepare with more energy and calories to train.  
 
 
A post shared by James “The Shed” Hollingshead (@hollingshead89)

The 2021 Olympia is currently scheduled to occur in September of next year when the annual contest usually occurs. If Hollingshead wins next year, he will be the first British man to attain the title of Mr. Olympia since Dorian Yates won six years in a row from 1992-97.
Featured Image: @hollingshead89 on Instagram

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